Optical Interference for Multiplexed, Label-free, and
Dynamic Biosensing: Protein, DNA and Virus Detection

4.13.2010  PRESENTERS: Selim Ünlü, PhD Professor of Electrical and Computer Engineering, Biomedical Engineering, and Physics, Associate Dean for Research and Graduate Programs, Associate Director, Center for Nanoscience and Nanobiotechnology, Boston University MODERATOR: Catherine Klapperich, PhD, Assistant Professor, Departments of Mechanical and Biomedical Engineering, Boston University Video not available.

Forum Abstract

Direct monitoring of primary molecular binding interactions without the need for secondary reactants would markedly simplify and expand applications of high-throughput label-free detection methods. Selim Ünlü's lab has developed a simple interferometric technique - Spectral Reflectance Imaging Biosensor (SRIB) - that monitors the optical phase difference resulting from accumulated biomolecular mass. Dynamic measurements were made at ~3 pg/mm2 sensitivity. The lab has also demonstrated simultaneous detection of antigens and antibodies in solution using corresponding probes on the SRIB surface as well as label-free measurements of DNA hybridization kinetics. The lab's biosensing technique does not depend on surface conformation of molecules and concentration changes in the solution. Thus, they have demonstrated that the measurements yield quantified and calibrated data on binding. Recently, they have simplified the instrumentation by utilizing discrete LED sources instead of a tunable laser, improved spatial resolutions and demonstrated capability in single pathogen detection.

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